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Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)
Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP IH |
|
| Catalog # | E3414-05H | | Cellular Localization | Cell Membrane | | Positive Control | Breast Carcinoma and human lung | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (paraffin): 1:50-1:100 using ABC method. Enzymatic predigestion of paraffin embedded tissue is recommended prior to immunostaining. | | Western Blot: 1:100-1:750 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8k76 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in 20 mM Tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, sodium azide. | | Purity | Purified | | Immunogen | HG9 cell line (small cell lung carcinoma). | | Specificity | This antibody reacts with two glycoproteins of 34 and 49kD present on the epithelial cells. It stains with simple epithelia and basal layers of stratified non-keratinized squamous epithelium. Species Crossreactivity: Crossreacts with Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | EpCam |
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