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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH
Catalog #E3414-05H
Cellular Localization Cell Membrane
Positive Control Breast Carcinoma and human lung
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:50-1:100 using ABC method. Enzymatic predigestion of paraffin embedded tissue is recommended prior to immunostaining.
Western Blot: 1:100-1:750
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8k76
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 20 mM Tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, sodium azide.
PurityPurified
ImmunogenHG9 cell line (small cell lung carcinoma).
SpecificityThis antibody reacts with two glycoproteins of 34 and 49kD present on the epithelial cells. It stains with simple epithelia and basal layers of stratified non-keratinized squamous epithelium. Species Crossreactivity: Crossreacts with Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEpCam


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