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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
During the First International Workshop on Small Cell Lung Cancer Antigens in London, April 1987, specific antibodies have been catagorized according to their reactivity patterns. This clone belongs to the so-called "cluster 2" reactivity pattern. It is unreactive with normal and malignant mesothelium and therefore especially useful for the detection of carcinoma cells in ascites or pleural effusions.
Catalog #E3414-06A
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen tissue and paraffin-embedded tissue after microwave treatment).
Recommended DilutionImmunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F264
ConcentrationNot determined
FormSupplied as a liquid.
PurityCulture supernatant
ImmunogenIsolated from small cell lung carcinoma-derived cell line.
SpecificityAntigen Recognized Human in Species (tested so far). Recommended for Bronchial tissue. Reacts with a 40kD membrane-associated protein present in most epithelia and in all lung carcinomas.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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