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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, Ep-CAM), EGP40) is expressed on the baso-lateral cell surface in most human simple epithelia and a vast majority of carcinomas. It is of recent interest as a tumor marker.
Catalog #E3414-10
ApplicationsImmunoprecipitation, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37ºC.
Positive ControlBreast carcinoma
Cellular Localization Cell membrane
Recommended DilutionImmunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate
Western Blotting: 1ug/ml
Immunohistochemistry: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.276
HostMouse
SourceHuman
Concentration100ug/ml
Form500ul antibody supplied as a liquid in a PBS buffer, pH 7.4, 0.1% BSA, 7.5mM sodium azide, 25% glycerol. Also available without BSA and azide. See E3414-10X.
PurityPurified by Protein G affinity chromatography.
ImmunogenSmall cell lung carcinoma cells.
SpecificityRecognizes a 40kD transmembrane epithelial glycoprotein (EGP40), also identified as human epithelial specific antigen (ESA) or epithelial cellular adhesion molecule (Ep-CAM). Species crossreactivity: Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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