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Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)
Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, Ep-CAM), EGP40) is expressed on the baso-lateral cell surface in most human simple epithelia and a vast majority of carcinomas. It is of recent interest as a tumor marker. | | | Catalog # | E3414-10 | | Applications | Immunoprecipitation, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37ºC. | | Positive Control | Breast carcinoma | | Cellular Localization Cell membrane | | Recommended Dilution | Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate | | Western Blotting: 1ug/ml | | Immunohistochemistry: 1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 0.N.276 | | Host | Mouse | | Source | Human | | Concentration | 100ug/ml | | Form | 500ul antibody supplied as a liquid in a PBS buffer, pH 7.4, 0.1% BSA, 7.5mM sodium azide, 25% glycerol. Also available without BSA and azide. See E3414-10X. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Small cell lung carcinoma cells. | | Specificity | Recognizes a 40kD transmembrane epithelial glycoprotein (EGP40), also identified as human epithelial specific antigen (ESA) or epithelial cellular adhesion molecule (Ep-CAM). Species crossreactivity: Does not react with rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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