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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, Ep-CAM), EGP40) is expressed on the baso-lateral cell surface in most human simple epithelia and a vast majority of carcinomas. It is of recent interest as a tumor marker.
Catalog #E3414-11
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Fomalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires digestion of tisssue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.
Optimal dilutions to be determined by the researcher.
Positive ControlBreast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.277
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3414-11X.
PurityPurified by Protein G affinity chromatography.
ImmunogenColon carcinoma LoVo cell line. MW of Antigen
SpecificityRecognizes a 40kD transmembrane epithelial glycoprotein (EGP40), also identified as human epithelial specific antigen (ESA) or epithelial cellular adhesion molecule (Ep-CAM). Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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