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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA & Azide Free)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
EGP40 is a 40kD transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
Catalog #E3414-11X
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 20 minutes at RT. Staining of formalin-fixed tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0, for 15 minutes at RT or 10 minutes at 37ºC.
Optimal dilutions to be determined by the researcher.
Positive ControlColon carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.277
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and sodium azide. See E3414-11.
PurityPurified by Protein G affinity chromatography.
ImmunogenColon carcinoma LoVo cell line. MW of Antigen: 40-43kD
Cellular LocalizationCell membrane
SpecificityRecognizes the human Epithelial Specific Antigen. Does not crossreact with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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