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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40, Ep-CAM, MK-1) (BSA/Azide Free)

Anti -Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, EGP40,
Ep-CAM, MK-1) (BSA/Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
Epithelial Specific Antigen (ESA, Epithelial Cellular Adhesion Molecule, Ep-CAM), EGP40) is expressed on the baso-lateral cell surface in most human simple epithelia and a vast majority of carcinomas. It reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
Catalog #E3414-12X
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Radioimaging and Immunohistochemistry (formalin/paraffin). Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1-2ug/ml for 30 minutes at room temperature.
Note: Staining of formalin-fixed tissues requires digestion of tissue sections with pepsin at 1mg/ml in Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.
Optimal dilutions to be determined by the researcher.
Positive ControlBreast carcinoma
Cellular LocalizationCell membrane
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.278
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide, See E3414-12.
PurityPurified by Protein G affinity chromatography.
ImmunogenMCF-7 human breast cancer cells.
SpecificityRecognizes a 40-43kD transmembrane epithelial glycoprotein (EGP40), also identified as human epithelial cellular adhesion molecule (Ep-CAM) or epithelial specific antigen (ESA). Species crossreactivity: Human. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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