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You are here:Home » Antibodies » Abs to Mucin » Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM,
Sialomucin or Episialin, MUC-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Catalog #E3414-15D
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG HRP
Recommended Positive Control Tissue Normal Epithelia
ApplicationsImmunohistochemistry: (frozen)- 1:10-1:40, (paraffin)- 1:10-1:40
Optimal working dilutions to be determined by researcher.
Immunohistochemistry This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2099
HostMouse
SourceHuman
ConcentrationAs reported
FormTissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityTissue culture supernatant.
ImmunogenDelipidated extract of human cream.
SpecificityRecognizes epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD.This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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