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You are here:Home » Antibodies » Abs to Mucin » Anti -Epithelial Membrane Antigen (EMA)

Anti -Epithelial Membrane Antigen (EMA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Catalog #E3414-15E
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:10 1:40 (frozen or paraffin). Paraffin sections do not require protein digestion pretreatment or antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9B58
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
PuritySupernatant.
ImmunogenDelipidated extract of human cream.
SpecificityRecognizes human epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD. This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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