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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Catalog #	E3414-15E
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:10 – 1:40 (frozen or paraffin). Paraffin sections do not require protein digestion pretreatment or antigen retrieval using heat treatment prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9B58
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Purity	Supernatant.
Immunogen	Delipidated extract of human cream.
Specificity	Recognizes human epithelial membrane antigen (EMA), a heavily glycosylated protein of 265-400kD. This antibody stains normal and neoplastic epithelium including adenocarcinomas from a range of sites, squamous and transitional cell carcinomas and mesotheliomas. Strong staining of glandular epithelium is seen, but staining of squamous epithelium may be rather patchy. Positive staining with this antibody demonstrates epithelial origin of tumors.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.