Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Mucin » Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM,
Sialomucin or Episialin, MUC-1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH
Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive.
Catalog #E3414-17
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:25-1:50 (streptavidin:biotin)
Formalin-fixed, paraffin-embedded tissue sections can be stained successfully but require heat treatment to restore antigenicity, before staining. This involves exposure of tissue sections to boiling 0.01M citrate buffer, pH 6.0 for 25-30 minutes or for 2-3 minutes at elevated pressure.
Optimal dilutions to be determined by the researcher.
SourceTissue culture supernatant
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No1.B.763
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide, before the addition of 40%. glycerol.
PurityPurifed by gel filtration following ammonium sulfate precipitation.
ImmunogenPurified human Epithelial Membrane Antigen
SpecificityRecognizes Human surface glycoprotein of approximately 54kD. Positive against all curent carcinoma cell lines.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links