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You are here:Home » Antibodies » Abs to Mucin » Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin, MUC-1)

Anti -Epithelial Membrane Antigen (EMA, CA15-3, Polymorphic Epithelial Mucin, PEM,
Sialomucin or Episialin, MUC-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH
Catalog #E3414-22
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin-embedded tissue; Proteolytic treatment not required).
Recommended DilutionImmunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F80
HostMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.
PurityAscites
ImmunogenIsolated from primary breast carcinoma cells.
SpecificityReactive with Epithelial Sialomucin, a mucous glycoprotein located mainly in the glycocalyx of most glandular epithelial cells and in carcinoma cells. Directed against a 400kD glycoprotein, corresponding to MAM-6. Occasionally, a very weak crossreactivity with sarcomas and brain tumors is observed. It does not react with normal colon or rectal epithelium and colorectal adenomas, whereas colorectal carcinomas stain positive. Does not crossreact with leukemias. Reactive with human. Recommended for Mamma carcinoma.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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