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You are here:Home » Antibodies » Abs to Ion Channel » Anti -Epithelial Sodium Channel, gamma (ENAC Gamma)

Anti -Epithelial Sodium Channel, gamma (ENAC Gamma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain. At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons. H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, and gamma, and delta-ENaC subunits), are expressed in epithelia of the vertebrate kidney, colon, lung, tongue, and brain. The ENaC subunits may form heterotrimeric active Na channel. ENaCs are involved in Na and water readsorption, and salty taste transduction) of vertebrate colon, lung, kidney and tongue. The superfamily of DEG/NaC proteins are characterized by intracellular N and C-termini, two TM domains, and a large extracellular loop. ENaC-beta subunit (mouse/rat 638 aa, human 640 aa), ENaC-gamma subunit (mouse 655 aa, rat 650 aa, and human 649 aa) are expressed in lung, kidney, colon and other tissues. ENaC-b from various species are ~80% identical, and only ~30% similarity with the ENaC-alpha ENaC-delta (human 638 aa) is expressed mainly in brain, pancreas, testis, and ovary. It is 27-30% homologous with the other ENaCs. It can associate with b, and g ENaC to form a functional channel.
Catalog #E3414-75D
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml
ELISA: 0.5-1ug/ml.
Immunohistochemistry: 2-20ug/ml. See references.
Optimal dilutions to be determined by the researcher.
Control Peptide E3414-75H Epithelial Sodium Channel, gamma, Rat Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration1mg/ml
FormSupplied as liquid in PBS, pH 7.4 and 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 14 AA Peptide sequence near the N-terminus of rat ENAC g (1). The peptide was coupled to KLH.
SpecificityRecognizes rat Epithelial Sodium Channel, gamma (ENAC Gamma). Species sequence homology: murine 100%, human 92%, rabbit 80%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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