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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Epithelial Specific Cell Surface Glycoprotein

Anti -Epithelial Specific Cell Surface Glycoprotein

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Epithelial Specific Cell Surface Glycoprotein is expressed in all human epithelial cell types except hepatocytes, gastric parietal cells, superficial layers in squamous stratified epithelia, sebaceous glands, cortical thymic epithelium, ovarian follicular epithelium, ependyma and plexus epithelium.
Catalog #E3415-10
ApplicationsSuitable for use in Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Formalin fixed, paraffin wax embedded tissues, provided short term formalin fixation is used (few hours). Subsequent trypsin treatment improves staining in a peroxidase test. Also suitable for use with frozen tissue sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.859
HostMouse
SourceHuman
Concentration50ug/ml
FormSupplied as 50ug lyophilized material with sodium azide. Reconstitute with 1ml distilled water.
PurityPurified by Protein A affinity chromatography.
ImmunogenEpithelium-Specific Cell Surface Glycoprotein
SpecificityReacts with a human surface glycoprotein of approximately 35kDa. All carcinoma cell lines tested are positive with strong reactivity for colon lines HT-29, WiDr and SW1116.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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