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You are here:Home » Antibodies » Abs to Infectious Disease EBV » Anti -Epstein Barr Virus, LMP (EBV)

Anti -Epstein Barr Virus, LMP (EBV)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E IH
Catalog #E3440-09A
Epitope Located in the internal part of the membrane-associated viral LMP.
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:25 to 1:50 for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by the researcher.
Positive Control EBV-positive lymphoblastoma
Cellular Localization Cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5G100
HostMouse
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityCulture supernatant
ImmunogenEBV-encoded recombinant latent membrane protein.
SpecificityReacts strongly with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. Also reacts with 25-50% of EBV-associated undifferentiated nasopharyngeal carcinomas and with Reed Sternberg cells in ~90% of EBV-associated Hodgkin’s disease cases. The cocktail recognizes distinct epitopes on the hydrophilic carboxyl region of LMP which is exposed to the cytosol. Species Crossreactivity: EBV-positive cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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