| Catalog # | E3440-11A |
| Applications | Suitable for use in Western Blot, Immunofluorescence and ELISA. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:10-1:50 (unboiled samples in 1mM DTT, 2% SDS). |
| Immunofluorescence: 1:10-1:50. Note: Three step amplification procedure must be used. |
| ELISA: 1:20-1:200 |
| Optimal dilutions to be determined by researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Immunofluorescence | 1. Slides/Fixation: |
| Cells should be suspended with PBS, pH 7.8, 200mM sodium chloride. |
| Air dried onto slides at RT. |
| Immediately fixed in-20ºC Methanol:Acetone (3:7). |
| Air dry slides. |
| Store @ 4ºC (<1 month) or at -20ºC (6-12 months) |
| 2. Staining: |
| Note: Three step amplification procedure must be used, i.e. Mab + goat anti-mouse IgG + Rabbit anti-goat labeled with FITC; or Mab + goat anti-mouse labeled with biotin + streptavidin labeled with FITC; or Mab + guinea pig complement + goat anti-guinea pig C3 labeled with FITC. |
| Each incubation should be for 45 minutes at 37ºC. |
| 3. Comments: |
| A two step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q1914 |
| Host | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Epstein Barr Virus, Nuclear Antigen. |
| Specificity | Specific for the Epstein Barr Nuclear Antigen (EBNA-1). |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
