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You are here:Home » Antibodies » Abs to Infectious Disease EBV » Anti -Epstein Barr Virus, Nuclear Antigen (EBV)

Anti -Epstein Barr Virus, Nuclear Antigen (EBV)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Catalog #E3440-11A
ApplicationsSuitable for use in Western Blot, Immunofluorescence and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1:10-1:50 (unboiled samples in 1mM DTT, 2% SDS).
Immunofluorescence: 1:10-1:50. Note: Three step amplification procedure must be used.
ELISA: 1:20-1:200
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunofluorescence1. Slides/Fixation:
Cells should be suspended with PBS, pH 7.8, 200mM sodium chloride.
Air dried onto slides at RT.
Immediately fixed in-20ºC Methanol:Acetone (3:7).
Air dry slides.
Store @ 4ºC (<1 month) or at -20ºC (6-12 months)
2. Staining:
Note: Three step amplification procedure must be used, i.e. Mab + goat anti-mouse IgG + Rabbit anti-goat labeled with FITC; or Mab + goat anti-mouse labeled with biotin + streptavidin labeled with FITC; or Mab + guinea pig complement + goat anti-guinea pig C3 labeled with FITC.
Each incubation should be for 45 minutes at 37ºC.
3. Comments:
A two step staining procedure is not sensitive enough to detect the EBNA. This is not a function of the monoclonal antibody, but rather a result of the small quantity of EBNA present in the cells.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1914
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenEpstein Barr Virus, Nuclear Antigen.
SpecificitySpecific for the Epstein Barr Nuclear Antigen (EBNA-1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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