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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)

Anti -ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The ErbB2 (HER2) proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1). ErbB2 does not have any known ligand; however, the kinase activity of ErbB2 can be activated without any ligand if it is overexpressed and by heteroassociation with other members of the ErbB family (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for breast cancer and other carcinomas. The therapeutic potential may result from directing ErbB2 to a c-Cblregulated proteolytic pathway. Tyrosine 877 of ErbB2 is homologous to pp60c-Src Tyr416 located in the kinase domain. Phosphorylation of this site may be involved in regulation of ErbB2 biological activity. Tyrosine 1248 of ErbB2 is one of the major autophosphorylation sites in this molecule. The phosphorylation of this site couples ErbB2 to the Ras- Raf-MAP kinase signal transduction pathway (5).
Catalog #E3451-26B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined.
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr877 of human ErbB2.
SpecificityRecognizes endogenous levels of human ErbB2 only when phosphorylated at tyrosine 877 at ~185kD. May crossreact with other ErbB family members (e.g., EGF receptor) when their homologous tyrosines are phosphorylated, but it does not crossreact with other tyrosine-phosphorylated proteins. Species sequence homology: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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