Anti -ErbB2, p185 (c-erbB2, HER2, neu Oncoprotein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH |
|
| The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments. | | | Catalog # | E3451-27 | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500 detects c-erbB-2 on 10ug of HEK293 lysates. | | Immunohistochemistry: Reported to detect c-erbB-2 in frozen and formalin/paraffin sections of rat breast. | | Immunoprecipitation: 4ug immunoprecipitated c-erbB-2 from 600ug of MCF-7 RIPA lysate and previously in A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysates. | | Optimal dilution determined by the researcher. | | Recommended Control | A431 cells, HEK293, Jurkat, MCF7 cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243- 1255 from the human c-erbB-2/HER-2/neu oncoprotein. | | Specificity | Recognizes human c-erbB-2 (185 kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Species Crossreactivity: monkey rat and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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