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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB2, p185 (c-erbB2, HER2, neu Oncoprotein)

Anti -ErbB2, p185 (c-erbB2, HER2, neu Oncoprotein)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
Catalog #E3451-27
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 detects c-erbB-2 on 10ug of HEK293 lysates.
Immunohistochemistry: Reported to detect c-erbB-2 in frozen and formalin/paraffin sections of rat breast.
Immunoprecipitation: 4ug immunoprecipitated c-erbB-2 from 600ug of MCF-7 RIPA lysate and previously in A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysates.
Optimal dilution determined by the researcher.
Recommended ControlA431 cells, HEK293, Jurkat, MCF7 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenA KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243- 1255 from the human c-erbB-2/HER-2/neu oncoprotein.
SpecificityRecognizes human c-erbB-2 (185 kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4. Species Crossreactivity: monkey rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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