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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)

Anti -ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19
Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell
Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface- bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2.
Catalog #E3451-27A
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml (ECL)
ELISA: 1:10,000-1:150,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11C99
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 100ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenPurified recombinant protein corresponding to aa23-652 at the extracellular domain of human ErbB2. (P04626).
SpecificityRecognizes human ErbB2. Does not react with human EGFR, Erbb3, or ErbB4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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