Anti -ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19
Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell
Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
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| HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface- bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Herstatin, as the product of alternative HER-2 transcript, retains intron 8. The herstatin mRNA is expressed in normal human fetal kidney and liver, but is at reduced levels relative to p185HER-2 mRNA in carcinoma cells that contain an amplified HER-2 gene. Herstatin appears to be an inhibitor of p185HER-2, because it disrupts dimers, reduces tyrosine phosphorylation of p185, and inhibits the anchorage-independent growth of transformed cells that overexpress HER-2. | | | Catalog # | E3451-27A | | Applications | Suitable for use in Western Blot and ELISA. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-2ug/ml (ECL) | | ELISA: 1:10,000-1:150,000 (10-100ng of control peptide/well). | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11C99 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% Trehalose. Reconstitute with 100ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | Purified recombinant protein corresponding to aa23-652 at the extracellular domain of human ErbB2. (Accession #P04626). | | Specificity | Recognizes human ErbB2. Does not react with human EGFR, Erbb3, or ErbB4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Metastatic Lymph Node Gene 19 Protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine Kinase-type Cell Surface Receptor HER2, p185erbB2, CD340, HER2, MLN19, NEU, NGL) |
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