Anti -ErbB2 (Receptor Tyrosine-protein Kinase erbB-2, Proto-oncogene Neu, Proto-oncogene
c-ErbB-2, p185erbB2, CD340, Kiaa3023, Neu)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
FC |
|
| ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a 185kD type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain aa23-653 of 1256 shares 85% and 94% aa identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 110kD soluble fragment of ErbB2 from the cell surface. ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. | | | Catalog # | E3451-27V | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 2.5ug labels 10e6 cells | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 12K88 | | Host | Rat | | Source | Mouse | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa23-653 from mouse ErbB2 expressed in NSO cell line. | | Specificity | Recognizes mouse ErbB2. Species sequence homology: Recombinant human ErbB2 recombinant mouse ErbB3, rmErbB4, and rmEGF R (<5%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
