Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian,
HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IC |
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| c-erbB-3/HER-3 is a member of the EGF receptor tyrosine kinase family. It is a kinase defective receptor which signals by forming heterodimers with other erb family members. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas and colon, although the significance is unclear because over-expression of c-erbB-3 has no effect on tumorigenic growth of cells. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. This antibody can be used to assess the contribution of the other erb family members co-expressed with c-erbB-3 to ligand-dependent signaling because it inhibits the binding of heregulin (NDF) to c-erbB-3/HER-3. | | | Catalog # | E3451-35 | | Applications | Suitable for use in Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunocytochemistry: 10ug/ml shows positive immunostaining for erbB-3 in MCF-7 cells fixed with 95% ethanol/5% acetic acid. | | Western Blot: 0.1-2ug/ml detects erbB-3 (~185kD) in RIPA lysates of human MCF-7 cells; previous lots have detected erbB-3 in MDA RIPA lysates. MCF-7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3451-35. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. | | Immunoprecipitation: 4ug antibody immunoprecipitates erbB-3 from human MDA and MCF-7 RIPA lysates. | | Immunohistochemistry: 1:50-1:100 dilution of a previous lot detected erbB-3 membrane staining in human normal and carcinoma breast tissue. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 0.T.39 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. Epitope maps between aa1295-1323 of human erbB-3 (NM_001982). | | Specificity | Recognizes human erbB-3 (~185kD). Does not crossreact with erbB-1 (EGFR) and erbB-2/HER-2. Species Sequence Homology: mouse, rat and bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3) |
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