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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian,
HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC
c-erbB-3/HER-3 is a member of the EGF receptor tyrosine kinase family. It is a kinase defective receptor which signals by forming heterodimers with other erb family members. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas and colon, although the significance is unclear because over-expression of c-erbB-3 has no effect on tumorigenic growth of cells. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. This antibody can be used to assess the contribution of the other erb family members co-expressed with c-erbB-3 to ligand-dependent signaling because it inhibits the binding of heregulin (NDF) to c-erbB-3/HER-3.
Catalog #E3451-35
ApplicationsSuitable for use in Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 10ug/ml shows positive immunostaining for erbB-3 in MCF-7 cells fixed with 95% ethanol/5% acetic acid.
Western Blot: 0.1-2ug/ml detects erbB-3 (~185kD) in RIPA lysates of human MCF-7 cells; previous lots have detected erbB-3 in MDA RIPA lysates. MCF-7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3451-35. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunoprecipitation: 4ug antibody immunoprecipitates erbB-3 from human MDA and MCF-7 RIPA lysates.
Immunohistochemistry: 1:50-1:100 dilution of a previous lot detected erbB-3 membrane staining in human normal and carcinoma breast tissue.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.T.39
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. Epitope maps between aa1295-1323 of human erbB-3 (NM_001982).
SpecificityRecognizes human erbB-3 (~185kD). Does not crossreact with erbB-1 (EGFR) and erbB-2/HER-2. Species Sequence Homology: mouse, rat and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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