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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian,
HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC IF
c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. c-erbB-3 is over-expressed in a variety of tumors including breast, stomach, pancreas, and colon. Heregulin & EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
Catalog #E3451-35B
MW of Antigen 185kD
Epitope Extracellular domain
Applications and Recommended DilutionsFlow Cytometry
Effect on Heregulin (NDF) Binding (Noninhibitory)
Effect on Tumor Growth (Non-inhibitory)
Immunofluorescence
Immunoprecipitation1 (Use Protein A; Ab 2ug/mg protein lysate)
Western Blotting (not suitable) The optimal dilution for a specific application should be determined by the investigator.
Positive Control T47D cells.
Cellular Localization Cytoplasmic and cell membrane
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F162
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3451-35BX
PurityPurified by Protein A affinity chromatography.
ImmunogenExtracellular fragment of recombinant human c-erbB-3/HER-3 oncoprotein
SpecificityDoes not block the binding of heregulin to c-erbB-31, has no effect on tyrosine kinase activity1, receptor internalization1, or tumorigenic growth of cells overexpressing the cerbB- 32. It is particularly well suited for immunoprecipitation of unoccupied as well as the occupied receptor1. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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