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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian,
HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP N
c-erb B-3/HER-3 is a member of the EGF receptor tyrosine kinase family. It is a kinase defective receptor which signals by forming heterodimers with other erb family members. c-erb B-3 is over-expressed in a variety of tumors including breast, stomach, pancreas and colon, although the significance is unclear because over-expression of c-erb B-3 has no effect on tumorigenic growth of cells. Heregulin and EGF stimulate tyrosine phosphorylation of c-erb B-3 to different extents. This antibody can be used to assess the contribution of the other erb family members co-expressed with c-erb B-3 to ligand-dependent signaling because it inhibits the binding of heregulin (NDF) to c-erb B-3/HER-3.
Catalog #E3451-36
ApplicationsSuitable for use in Neutralization and Immunoprecipitation.
Other applications not tested.
Recommended DilutionsNeutralization: 10ug/ml blocked heregulin mediated activation of c-erb B-3/HER-3 receptor in MCF-7 cells. Serum starved MCF-7 cells were stimulated with heregulin in the presence or absence of the neutralizing E3451-36. was immunoprecipitated with 5ug E3451-35. Immune complexes, split into duplicate samples, were resolved by electrophoresis and transferred to nitrocellulose. Duplicates were then treated with 1ug/ml P4078-05 or 1ug/ml E3451-35. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Immunoprecipitates unoccupied c-erb B-3/HER-3 receptors.
Optimal dilutions to be determined by the researcher.
HybridomaP3X63Ag8U1 (p3U1) cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.40
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4.
PurityPurified by Protein G affinity chromatography.
ImmunogenExtracellular domain of recombinant human c-erb B-3/HER-3 oncoprotein.
SpecificityRecognizes human c-erb B-3 (Mr~185kD), but not EGF receptor, c-erb B-2/HER-2 or c-erb B-4/HER-4. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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