Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian,
HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP N |
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| c-erb B-3/HER-3 is a member of the EGF receptor tyrosine kinase family. It is a kinase defective receptor which signals by forming heterodimers with other erb family members. c-erb B-3 is over-expressed in a variety of tumors including breast, stomach, pancreas and colon, although the significance is unclear because over-expression of c-erb B-3 has no effect on tumorigenic growth of cells. Heregulin and EGF stimulate tyrosine phosphorylation of c-erb B-3 to different extents. This antibody can be used to assess the contribution of the other erb family members co-expressed with c-erb B-3 to ligand-dependent signaling because it inhibits the binding of heregulin (NDF) to c-erb B-3/HER-3. | | | Catalog # | E3451-36 | | Applications | Suitable for use in Neutralization and Immunoprecipitation. | | Other applications not tested. | | Recommended Dilutions | Neutralization: 10ug/ml blocked heregulin mediated activation of c-erb B-3/HER-3 receptor in MCF-7 cells. Serum starved MCF-7 cells were stimulated with heregulin in the presence or absence of the neutralizing E3451-36. was immunoprecipitated with 5ug E3451-35. Immune complexes, split into duplicate samples, were resolved by electrophoresis and transferred to nitrocellulose. Duplicates were then treated with 1ug/ml P4078-05 or 1ug/ml E3451-35. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunoprecipitation: Immunoprecipitates unoccupied c-erb B-3/HER-3 receptors. | | Optimal dilutions to be determined by the researcher. | | Hybridoma | P3X63Ag8U1 (p3U1) cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 0.T.40 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Extracellular domain of recombinant human c-erb B-3/HER-3 oncoprotein. | | Specificity | Recognizes human c-erb B-3 (Mr~185kD), but not EGF receptor, c-erb B-2/HER-2 or c-erb B-4/HER-4. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -ErbB3 (ERBB3, v-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 avian, HGNC:3431, ErbB-3, HER3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3) |
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