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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB4 (c-erbB4, HER4)

Anti -ErbB4 (c-erbB4, HER4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). Down regulation of ErbB-4 in estrogen receptor-positive cancer cells inhibits proliferation.
Catalog #E3451-40A
MW of Antigen 185kD
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Internalization of c-erbB-4 and stimulation of tyrosine kinase activity. Not suitable for use in Western Blot and Inhibition of receptor-heregulin binding1. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate (Use Protein G). (Unoccupied as well as the occupied c-erbB-4 receptor).
Optimal dilutions to be determined by the researcher.
Positive ControlT47D or MDA-MB-453 cells
Cellular LocalizationCell membrane and cytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3F166
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3451-40AX
PurityPurified by Protein G affinity chromatography.
ImmunogenExtracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein
SpecificityRecognizes human c-erbB-4/HER-4 oncoprotein. Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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