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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB4 (c-erbB4, HER4) (BSA & Azide Free)

Anti -ErbB4 (c-erbB4, HER4) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
c-erbB4 is the fourth member of class I receptor kinase family
(ErbB). HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDA-MB-330 and MDA-MB-361. Expression of erbB4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468 and SKBR-3.
Catalog #E3451-40BX
ApplicationsSuitable for use in Flow Cytometry and Inhibition of heregulin
(NDF) binding. Not suitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionInhibition of Heregulin (NDF) Binding: Order Ab without sodium azide
Immunoprecipitation: Only Immunoprecipitates the unoccupied receptor.
Optimal dilutions to be determined by the researcher.
Positive ControlT47D or MDA-MB-453 cells
Cellular LocalizationCytoplasmic and cell membrane
EpitopeExtracellular domain
Source Ascites
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F167
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See E3451-40B.
PurityPurified by Protein A affinity chromatography.
ImmunogenExtracellular domain of recombinant human c-erbB4/HER4 oncoprotein
SpecificityRecognizes human ErbB4 (c-erbB4, HER4) at 185kD. Inhibits the binding of heregulin (NDF) to c-erbB4. Does not effect tyrosine kinase activity or internalization of c-erbB4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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