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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB4 (c-erbB4, HER4) (BSA & Azide Free)

Anti -ErbB4 (c-erbB4, HER4) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
c-erbB-4 is the fourth member of class I receptor kinase family (ErbB). HER-4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDA-MB-330 and MDA-MB-361. Expression of erbB-4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3
Catalog #E3451-40CX
MW of Antigen 185kD
Epitope aa1249-1264
Applications and Recommended DilutionsImmunohistochemistry (Formalin/paraffin) (Ab at 5-10ug/ml for 30 min at RT) * [No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues] The optimal dilution for a specific application should be determined by the investigator.
Positive Control Breast carcinoma or Skeletal muscle.
Cellular Localization Cytoplasmic and cell membrane
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No3F168
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See E3451-40C
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide corresponding to aa1249-1264 (Cys-RSTLQHPDYLQEYST) from the human c-erbB-4/HER-4 oncoprotein.
SpecificityRecognizes ErbB4 (c-erbB4, HER4). Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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