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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB4 (c-erbB4, HER4)

Anti -ErbB4 (c-erbB4, HER4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
c-erbB-4 is the fourth member of class I receptor kinase family. HER-4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDA-MB-330 and MDA-MB-361. Expression of erbB-4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3.
Catalog #E3451-40F
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:50 for 60 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlBreast carcinoma, T47D cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide from C-terminus of human c-erbB-4 protein. Cellular Localization: Cytoplasmic and cell membrane.
SpecificityRecognizes human c-erbB4, Mr 185kD. Epitope: C-terminal.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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