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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -ErbB4 (c-erbB4, HER4)

Anti -ErbB4 (c-erbB4, HER4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
erbB4/HER4 is the fourth member of the human epidermal growth factor receptor (EGFR) family that is frequently overexpressed in a variety of aggressive epithelial carcinomas. Its extracellular domain is most similar to the orphan receptor HER3, whereas its cytoplasmic kinase domain exhibits 79% and 77% identity with EGFR and HER2, respectively. HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain and cerebellum. Breast tumor cell lines MDA-MB-453, BT-474, T47-D and H3396 have been shown to have the highest levels of erbB-4 mRNA. Intermediate levels have been seen in MCF-7, MDA-MB-330 and MDA-MB-361 cell lines. erbB-4 expression has been shown to be low or absent in MDA-MB-231, MDA-MB-157, MDA-MB-468 and SKBR-3 cell lines.
Catalog #E3451-41
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1-4ug/ml detects erbB4/HER4 in RIPA lysates from MCF-7 and MDA-MB-453 cells.
Immunoprecipitation: 4ug immunoprecipitates erbB4/HER4 from 500ug of MCF-7 RIPA lysate.
Immunoprecipitation/Western Blot Analysis: MCF-7 cell lysate was immunoprecipitated with 4ug of E3451-41. The immune complex was subsequently resolved by electrophoresis and transferred to nitrocellulose. Proteins were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.09% sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1285-1308 at the C-terminus of human erbB4/HER4, conjugated with KLH.
SpecificityRecognizes human erbB4/HER4, Mr 185kD. Does not recognize erbB1(EGFR), erbB2 or erbB3. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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