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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Erythrocytes

Anti -Erythrocytes


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Clone Host Grade Applications
Polyclonal Rabbit Highly Purified
Catalog #E3453-46A
ApplicationsSuitable for use to sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes. Other applications have not been tested.
Recommended Dilutions
Haemolytic complement assays: 1:60 (Haemolytic titre) at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement and 2% RBC, not less than 1:500 when tested with bovine erythrocytes.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
PurityPurified by DEAE chromatography.
ImmunogenWashed whole erythrocytes were prepared from fresh blood and used for intravenous immunization.
SpecificityRecognizes bovine Erthyrcytes. Homologous proteins of different species frequently share antigenic determinants.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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