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You are here:Home » Growth Factors, Cytokines » Growth Factors-EPO » Erythropoietin, alpha, Recombinant, Human (Powder) (EPO, EPO-a, EPO-alpha, Epoetin, EP, MGC138142)

Erythropoietin, alpha, Recombinant, Human (Powder) (EPO, EPO-a, EPO-alpha, Epoetin, EP,
MGC138142)

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Specifications

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Catalog #E3455-07A
Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166aa and having a predicted molecular mass of 21kD and apparent glycosylated molecular mass of 36-40kD.
Specific ActivityMeasured using Normocythaemic mice and was found to be 150,000 IU/mg
Amino Acid SequenceAPPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight36-40kD
SourceChinese hamster ovary (CHO) cells
Purity98% by RP-HPLC and SDS-PAGE. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from a sodium citrate, sodium chloride, citric acid buffer. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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