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You are here:Home » Molecular Biology » MB-Growth Factors-EPO » Erythropoietin, beta, Recombinant, Human (EPO)

Erythropoietin, beta, Recombinant, Human (EPO)


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Recombinant Human Erythropoietin-beta is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21kD and apparent glycosylated molecular mass of 30.4kD.
Catalog #E3455-09
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityErythropoietin is fully biologically active when compared to standard. The Specific Activity was measured by Normocyth-aemic mice and was found to be 1.2x10e5IU/mg.
Endotoxin 0.1ng/ug (IEU/ug) of EPO-b.
ReconstitutionReconstitute the lyophilized EPO-b in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceCHO cells
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
FormSupplied as a lyophlilzed powder in sodium phosphate dibasic, sodium phosphate monobasic buffer, 60% sodium chloride, 0.6% calcium chloride and 0.004% Tween 20.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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