Erythropoietin

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

P01588

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC N WB

Crossreactivity

Accession #

NP_000790.2

Gene ID

2056

Shipping Temp

Blue Ice

Storage Temp

-20°C

Erythropoietin Precursor, Epoetin

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.

Applications

Suitable for use in ELISA, Functional Assays, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions

Immunohistochemistry (Paraffin): 15ug/ml ELISA: 0.5-1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to human Erythropoietin expressed in CHO cells.

Form

Supplied as a liquid in PBS, pH 7.2, <5% trehalose.

Purity

Purified by Protein A affinity chromatography. |Endotoxin: <0.1EU/ug (LAL)

Specificity

Recognizes human Erythropoietin.

References

1. Krystal, G., et al. (1983) A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice. Exp. Hematol. 11: 649-660. 2. Kessler, S.W., et al. (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 115: 1617-1624.

USBio References

No references available

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E3455-10R Rabbit Anti-Erythropoietin (EPO, EPO alpha, EP, MGC138142, MVCD2)

Clone Type

Host

Source

Swiss Prot

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

Shipping Temp

Storage Temp

Erythropoietin Precursor, Epoetin

References

USBio References

Conjugates

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