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You are here:Home » Antibodies » Antibodies-Carbohydrates, Glycoproteins » Anti -Endothelial Cell-Specific Adhesion Molecule (ESAM)

Anti -Endothelial Cell-Specific Adhesion Molecule (ESAM)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Endothelial Cell-Specific Adhesion Molecule (ESAM) is a 55kD type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% aa sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.
Catalog #E3486-50A
ApplicationsSuitable for use in Direct ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 8-25ug/ml in frozen mouse liver, thymus and intestine tissue sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H117
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa30-248 at the extracellular domain of mouse ESAM expressed in NSO cells (Q925F2)
SpecificityRecognizes mouse ESAM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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