Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified IF

Escherichia coli is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885,and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
Catalog #	E3500-04M
Applications	Suitable for use in Immunofluorescence. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 1:10-1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified
Immunogen	Raised against a mixture of all antigenic serotypes
Specificity	Recognizes Escherichia Coli and is broadly reactive with all somatic and capsular (”O” and “K”) antigenic serotypes. Is unabsorbed and will crossreact with related enterobacteriaceae. Unconjugated antibody has been used to detect serotypes O157:H7, O20, O125, O55, O111 and K12.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.