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You are here:Home » Antibodies » Abs to Infectious Disease E. coli » Anti -Escherichia coli, O157:H7, Pathogenic (E. coli)

Anti -Escherichia coli, O157:H7, Pathogenic (E. coli)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, or by preventing the establishment of pathogenic bacteria within the intestine. E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
Catalog #E3500-25
ApplicationsSuitable for use in Agglutination and Solid Phase Immunoassay.
Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Matched PairCapture: E3500-25
Detection: E3500-26
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG3
Clone No8.F.101
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenE. coli O157:H7 LPS, O-polysaccharide moiety.
SpecificityRecognizes the E. coli serotype O157. Gives a single precipitin arc with the alkaline-treated E. coli LPS and O polysaccharide. Does not crossreact react with E. coli serotypes O111, O125, O20, O55 & K12.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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