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You are here:Home » Antibodies » Abs to Infectious Disease E. coli » Anti -Escherichia coli, J5 LPS (E. coli)

Anti -Escherichia coli, J5 LPS (E. coli)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IF
The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
Catalog #E3500-28J
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No10C141
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenE. coli J5 cells
SpecificityRecognizes E. coli J5 LPS. Species crossreactivity: K. pneumoniae, S. sonnei and S. typhimurium.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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