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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IP IF

Escherichia coli (E. coli) is a Gram negative bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for costly product recalls.[1][2] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,[3] or by preventing the establishment of pathogenic bacteria within the intestine.[4][5] E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for fecal contamination.[6][7] The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of metagenics, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E. coli was discovered by German pediatrician and bacteriologist Theodor Escherich in 1885,[6] and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.[8]
Catalog #	E3500-39
Applications	Suitable for use in ELISA, Immunofluorescence and Immunoprecipitation. Also suitable for conjugation purposes. Other applications not tested.
Recommended Dilution	ELISA: 1:10-1:100; Functions as a capture antibody, detector antibody and pairs with itself.
	Immunofluorescence: 1:5-1:25
	Conjugation: Conjugates with colloidal gold
	Optimal dilutions to be determined by the researcher.
	Lateral Flow Matched Pairs:
	E3500-39
	E3500-42
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	5E275
Host	Mouse
Concentration	~0.05mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins have been added.
Purity	~90% Purified by protein A chromatography.
Immunogen	Escherichia coli
Specificity	Specific for E. coli serotype O157 by immunofluorescence. Immunoprecipitates LPS of O157
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.