You are here:Home
»
Antibodies
»
Abs to Infectious Disease E. coli
»
Anti -Escherichia coli, O157 (E. coli) (FITC)
Anti -Escherichia coli, O157 (E. coli) (FITC)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IP IF |
|
| E. coli O157:H7 is an enterohemorrhagic strain of the bacterium E. coli and a cause of foodborne illness. It is a gram-negative rod-shaped bacterium. The letter "O" (not a zero) in the name refers to the somatic antigen number, whereas the "H" refers to the flagella antigen. E. coli O157:H7 is markedly different from other pathogenic E. coli. In particular, the O157:H7 serotype is negative for invasiveness (sereny test), elaborates no colonization factors (CFA/I or CFA/II), doesn't produce heat stable or heat labile toxins and is non-hemolytic. In addition, E. coli O157:H7 is usually sorbitol negative whereas 93% of all E. coli ferment sorbitol. E. coli O157:H7 also lacks the ability to hydrolyze 4-methylumbelliferyl-beta-D-glucuronide (MUG) and does not grow at 45°C in the presence of 0.15% bile salts. Because of the latter characteristic this serotype cannot be isolated by using standard fecal coliform methods that include incubation at 45°C. | | | Catalog # | E3500-39A | | Applications | Suitable for use in Immunofluorescence, ELISA and Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | Immunofluorescence: 1:5-1:20 Acetone fixation of the antigen source is recommended prior to staining. | | ELISA: 1:5-1:20 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG3 | | Clone No | 5E275 | | Host | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Escherichia coli (E coli) serotype O157 | | Specificity | Recognizes E. coli serotype O157. Immunoprecipitates LPS of O157:H7. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
