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You are here:Home » Antibodies » Abs to Infectious Disease E. coli » Anti -Escherichia coli, O157 (E. coli) (FITC)

Anti -Escherichia coli, O157 (E. coli) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IF
E. coli O157:H7 is an enterohemorrhagic strain of the bacterium E. coli and a cause of foodborne illness. It is a gram-negative rod-shaped bacterium. The letter "O" (not a zero) in the name refers to the somatic antigen number, whereas the "H" refers to the flagella antigen. E. coli O157:H7 is markedly different from other pathogenic E. coli. In particular, the O157:H7 serotype is negative for invasiveness (sereny test), elaborates no colonization factors (CFA/I or CFA/II), doesn't produce heat stable or heat labile toxins and is non-hemolytic. In addition, E. coli O157:H7 is usually sorbitol negative whereas 93% of all E. coli ferment sorbitol. E. coli O157:H7 also lacks the ability to hydrolyze 4-methylumbelliferyl-beta-D-glucuronide (MUG) and does not grow at 45°C in the presence of 0.15% bile salts. Because of the latter characteristic this serotype cannot be isolated by using standard fecal coliform methods that include incubation at 45°C.
Catalog #E3500-39A
ApplicationsSuitable for use in Immunofluorescence, ELISA and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:5-1:20 Acetone fixation of the antigen source is recommended prior to staining.
ELISA: 1:5-1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG3
Clone No5E275
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenEscherichia coli (E coli) serotype O157
SpecificityRecognizes E. coli serotype O157. Immunoprecipitates LPS of O157:H7.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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