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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Estradiol 17b hemisuccinate (BSA) (1,3,5(10)-Estratrien-3,17b-diol-17hemisuccinate BSA)

Estradiol 17b hemisuccinate (BSA) (1,3,5(10)-Estratrien-3,17b-diol-17hemisuccinate BSA)

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Specifications

Catalog #E3550-14
Nomenclature1,3,5(10)-Estratrien-3,17b-diol-17hemisuccinate-BSA
Molecular Ratio Steroid 35
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 0.5mg/ml gives 75% maximal amplitude
Optimal dilutions to be determined by the researcher.
Solubility of BSA conjugatesHaptens with ~30 steroids per molecule are designed for raising antibodies. They are virtually insoluble in water and buffer without detergents. The lack of solubility in water arises from both the steroid content and crosslinking. We suggest solubilization with a detergent with vigorous stirring, for example Freund's adjuvant (which contains a detergent) works well. Place the steroid onto the solution, not the other way round.
Dissolving BSA conjugates in water for cell culture experiments with molar ratios of greater than 10 moles of steroid per mole of BSA is almost impossible.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at 20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight65,000+
SourceSynthesized from BSA and 17-hemisuccinate-Estradiol.
Melting Point93-95°C
Rotation+42.1°
Molecular Ratio Steroid35
FormSupplied as a White to yellow crystalline powder. powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names1,3,5(10)-Estratrien-3,17b-diol-17hemisuccinate -BSA


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