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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
The Estrogen Receptors (ER), ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are also found in the plasma membrane (8). Steroid hormone receptors are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain and a steroid binding domain. The DNA binding domain is separated from the steroid binding domain by a hinge region. The DNA binding domain has a high degree of homology amongst the various steroid hormone receptors, followed by the steroid binding domain. The N-terminus is the most divergent region among members of the steroid receptor family. A diagram showing the relative location of these domains is shown below: N H COOH + 1 595 2 S teroid DNA TA F h ing e H um an E strog en R e ce p to r 287 300 e p ito p e 302 180 263 Estrogen has pleiotropic effects on the growth, development and maintenance of diverse tissues (9). The ERalpha and ERbeta isoforms have similar structures, but show differences in tissue expression and can mediate overlapping but different sets of biological functions (10). ERalpha is predominantly expressed in breast, uterus, cervix, vagina, and other target organs. ERbeta has a more limited expression pattern and is primarily detected in ovary, prostate, testis, spleen, lung, hypothalamus, and thymus (12). Many other ER-interacting proteins confer distinct functionalities on the ERs, including activation and repression of transcription, integration of intracellular signalling pathways and control of cell cycle progression. The ERs function by integrating multiple signals from ligands and intracellular signalling pathways (9). ER knockout mice have been used to study the role of ERs with respect to brain degeneration, osetoporosis, cardiovascular diseases and obesity (11).
Catalog #E3559-02B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:250
Immunoprecipitation: 15ug/ml
Optimal dilutions to be determined by researcher.
NoteFor use in immunoprecipitation, we recommend that protein A Sepharose be
used as immunoabsorbent with a bridging rabbit anti-mouse IgG to ensure
efficient binding of the antibody.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4E72
HostMouse
SourceHuman
FormSupplied as a liquid in PBS pH 7.2, 0.1mM PMSF, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenA 14 residue synthetic peptide RAANLWPSPLMIKR based on the human estrogen receptor (residues 287-300) was synthesized and the peptide coupled to KLH.
SpecificitySRA-1000 recognizes an epitope in the hinge region of the estrogen receptor. Binding specificity was confirmed in peptide inhibition studies with the intact protein. The antibody reacts with ER in human breast cancer cells as well as with mouse, rat and calf ER. The antibody does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor. In immunoprecipitation studies, this antibody has been shown to only IP the active receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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