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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER) (BSA & Azide Free)

Anti -Estrogen Receptor (ER) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy
Catalog #E3564-75X
ApplicationsSuitable for use in Gel Supershift, Immunoprecipitation and Affinity Purification. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate; Use Protein G, Native and denatured.
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,l
Clone No0.N.282
HostMouse
SourceBovine
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 40% glycerol. Also available with BSA and azide. See cat# E3564-75.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, SDS-denatured calf uterus estrogen receptor. Epitope
SpecificityRecognizes bovine Estrogen Receptor (ER) at 67kD. The epitope maps between residues 125-165. On chymotryptic cleavage, recognizes only the undigested ER indicating that its epitope is dismembered by digestion. Species Crossreactivity: Human, rabbit, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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