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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.
Catalog #E3564-78A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8A144
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
PurityPurified immunoglobulin
ImmunogenNi-NTA purified truncated recombinant ER expressed in E. coli strain BL21 (DE3)
SpecificityRecognizes truncated ER recombinant protein/MCF-7 cell lysate (WB), ER recombinant protein (ELISA).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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