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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Catalog #E3564-85
ApplicationsSuitable for use in Immunohistochemistry (paraffin tissues) and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: :50-1:100 for 2 hrs at RT
Immunohistology (Formalin/paraffin): 1:100-1:200 for 30 min at RT. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control MCF-7 cells. Breast Carcinoma.
Cellular Localization Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1 + IgG1,k
Clone No5G102
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityCulture supernatant
ImmunogenRecombinant human estrogen receptor protein.
SpecificityRecognizes Estrogen Receptor. Strongly stains the nucleus of epithelial cells in breast carcinomas. Species Crossreactivity: Human and Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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