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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (BSA & Azide Free) (ER)

Anti -Estrogen Receptor (BSA & Azide Free) (ER)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
ER protein exhibits six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. It is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors may improve response to anti-estrogen therapy.
Catalog #E3564-89X
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT
ELISA: Use as a reporter antibody
Optimal dilutions to be determined by the researcher
Positive ControlMCF-7 cells, Breast Carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5G106
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See E3564-89.
PurityPurified by Protein G affinity chromatography.
ImmunogenEstrogen receptor purified from MCF-7 cells.
Cellular LocalizationNuclear
SpecificityRecognizes human Estrogen Receptor alpha at ~67kD.
Species CrossreactivityRat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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