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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, phosphorylated (Ser118) (ER)

Anti -Estrogen Receptor, phosphorylated (Ser118) (ER)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #E3565-03
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: A 1:2000-1:500 dilution of this lot detected phospho-ERa (Ser118) in RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant ERa phosphorylated in vitro with MAPK2/Erk2.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 100uM EDTA, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …QLpSPF… in which pS corresponds to phosphoserine at residue 118 of
human estrogen receptor alpha. The immunizing sequence has 12/13 identical amino acids in camel, mouse, porcine and syrian hamster.
SpecificityRecognizes phospho-ERa (Ser118), Mr ~66kD and a splice variant of 60kD. Species Crossreactivity: Human. Predicted to crossreact with camel, mouse, porcine and syrian hamster based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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