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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, phosphorylated (Ser167) (ER)

Anti -Estrogen Receptor, phosphorylated (Ser167) (ER)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Catalog #E3565-04
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation/Immunoblot Analysis: A 1:500-1:1000 dilution of this lot detected phospho-estrogen receptor a (Ser167) in RSK1/MAPKAP-K1a treated estrogen receptor a immunoprecipitates from MCF-7 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 5mg/ml BSA) and 30% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …LApSTN… in which pS corresponds to phosphoserine at residue 167 of
human estrogen receptor alpha. The immunizing sequence has 11/12 identical amino acids in canine, goat, porcine and sheep.
SpecificityRecognizes phospho-estrogen receptor a (Ser167), Mr 66kD. Species Crossreactivity: Human. Predicted to crossreact with canine, goat, porcine and sheep based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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