Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Catalog #E3565-06D
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-1.0ug/ml for 2hrs at RT
Immunohistochemistry (formalin/paraffin): 0.4-0.8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cells, Breast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F173
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3565-06DX
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human estrogen receptor protein. Epitope: N-terminal domain.
SpecificityRecognizes human Estrogen Receptor. Strongly stains the nucleus of epithelial cells in breast carcinomas. Species Crossreactivity: monkey, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links