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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.
Catalog #E3565-06E
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 2.5-5ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:62.5-1:125 for 20 minutes at RT. (Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.)
Immunoprecipitation: 10ug/mg protein lysate. Denatured verified, use protein A.
Optimal dilutions to be determined by the researcher.
Positive Control
MCF-7 cells. Breast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See E3565-06EX
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide derived from the C-terminal of human estrogen receptor, alpha protein. Cellular Localization: Nuclear.
SpecificityRecognizes human Estrogen Receptor, 67kD. Species Crossreactivity: mouse and hamster. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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