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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor (ER) (BSA & Azide Free)

Anti -Estrogen Receptor (ER) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy
Catalog #E3565-06EX
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 10ug/mg protein lysate Use Protein A
Western Blot: 2.5-5ug/ml for 2hrs at RT
Immunohistochemistry: (Formalin/paraffin): 1:500 for 20 minutes at RT using the LP system, for 30 minutes at RT Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution to be determined by researcher.
Positive ControlMCF-7 cells. Breast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See E3565-06E
PurityPurified by Protein A affinity chromatography.
ImmunogenA synthetic peptide derived from the C-terminus of human estrogen receptor, alpha protein Epitope
SpecificityRecognizes human Estrogen Receptor (ER). Species Crossreactivity: Mouse and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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