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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor-a (ER, ER-alpha, Estradiol Receptor, Nuclear Receptor Subfamily 3 Group A Member 1, ESR1, ESR, NR3A1)

Anti -Estrogen Receptor-a (ER, ER-alpha, Estradiol Receptor, Nuclear Receptor Subfamily 3
Group A Member 1, ESR1, ESR, NR3A1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Estrogen receptor-a is a ligand activated, transactivating protein. The wild type estrogen receptor-a coded by a 6.6kb mRNA, comprised of five functional domains derived from eight exons. The three domains of the protein which show the greatest degree of sequence homology are the A domain which, in concert with the B domain, contains the transactivation function (AF-1), the C domain which contains two zinc-fingers and is responsible for DNA binding and the E domain which is responsible for ligand binding as well as the ligand inducible transactivation function (AF-2). Amplification of the protein is a common mechanism in proliferative breast disease. Estrogen receptor-a IVS1-397T/C polymorphism is associated with risk of myocardial infarction and other cardiovascular disease in both men and women. Human estrogen receptor-a gene is mapped to chromosome 6q25.1.
Catalog #E3565-09H
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlMCF7 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human Estrogen Receptor-a around the phosphorylation site of serine118 (Q-L-SP-P-F).
SpecificityRecognizes human Estrogen Receptor-a. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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