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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ERa)

Anti -Estrogen Receptor, alpha, phosphorylated (Ser118) (ERa)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IF
Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha. The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1).
Catalog #E3565-09L
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Not recommended for use in FACS or Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:10,000
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (IC): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlsMCF7 cell lysate human breast adenocarcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k168
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic phosphopeptide from the region around Ser118 of human Estrogen Receptor, alpha.
SpecificityRecognizes human ERa when phosphorylated at Ser118 at ~66kD. Does not ract with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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