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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -Estrogen Receptor, alpha (ERa)

Anti -Estrogen Receptor, alpha (ERa)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC FC Gel
Estrogen Receptor alpha is a 65kD protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.
Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
Catalog #E3565-09T
ApplicationsSuitable for use in Gel Supershift, Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml, detects an ~67kD protein representing ER alpha from MCF-7 cell extract.
Immunocytochemistry: 10ug/ml
Immunohistochemistry (Paraffin-embedded sections): 5ug/ml, staining of ER alpha in mouse Leydig cells results in both nuclear and cytoplasmic staining.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8k119
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurifed by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to amino acid residues 247-261 [(247)EVGMKGGIRKDRRG(261)] of the ER alpha DNA binding domain. Cellular Localization: Nuclear.
SpecificityRecognizes both the steroid occupied and unoccupied forms of human estrogen receptor (ER) alpha at ~65kD. Does not crossreact with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor. Species Crossreactivity: rat, mouse, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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